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Reconstitution Guide

Everything you need to reconstitute peptides correctly. Bacteriostatic water is included free with every order.

What's in Every PEPTOVA Order
✓ Peptide vial(s)
✓ 10ml bacteriostatic water
✓ Batch # on every vial
✓ COA verify link

You'll also want: insulin syringes (1ml, 29–31 gauge), alcohol swabs.

Step-by-Step Reconstitution

01
Let vial reach room temperature

Remove from refrigerator 15–20 minutes before reconstituting. Cold vials can cause the powder to clump and dissolve unevenly.

02
Swab both vial tops with alcohol

Wipe the rubber stopper on both the peptide vial and the bacteriostatic water vial. Let dry 10–15 seconds.

03
Draw bacteriostatic water into syringe

Draw your desired amount of bac water (see table below). Use a clean insulin syringe.

04
Inject water slowly down the inner vial wall

Critical: Insert the needle and tilt it so water runs slowly down the glass wall — not directly onto the powder. This prevents foaming and protein denaturation.

05
Swirl gently — never shake

Gently roll the vial between your palms until clear. Do not shake — agitation degrades peptide bonds. If powder doesn't dissolve, wait 5 minutes and swirl again.

06
Refrigerate at 2–8°C — use within 28 days

Reconstituted peptides are stable at 2–8°C for up to 28 days. Label the vial with the date. For long-term storage, keep lyophilized (unreconstituted) at −20°C.

Reconstitution Volume Reference

How much bac water to add per vial based on the concentration you want.

Vial Size Add Bac Water Final Concentration Per Unit (IU syringe)
2mg vial1 ml2 mg/ml (2000 mcg/ml)10 IU = 200 mcg
5mg vial2.5 ml2 mg/ml (2000 mcg/ml)10 IU = 200 mcg
5mg vial1 ml5 mg/ml (5000 mcg/ml)10 IU = 500 mcg
10mg vial2 ml5 mg/ml (5000 mcg/ml)10 IU = 500 mcg
10mg vial1 ml10 mg/ml (10,000 mcg/ml)10 IU = 1000 mcg

IU = Units on a standard 100-unit insulin syringe. 10 IU = 0.1ml.

Compound-Specific Notes

BPC-157 (5mg) Most Popular

Add 2.5ml bac water → 2mg/ml. Stable in solution at 2–8°C for 28 days. Lyophilized powder stable at −20°C for 24+ months. Clear to slightly yellow solution is normal.

TB-500 / Thymosin Beta-4 (5mg, 10mg) Recovery

5mg: Add 1ml bac water → 5mg/ml. 10mg: Add 2ml → 5mg/ml. TB-500 dissolves slowly — allow 5–10 minutes of gentle swirling. Do not shake. Refrigerate after reconstitution.

CJC-1295 No DAC (2mg) + Ipamorelin (5mg) GH Axis

CJC-1295 No DAC 2mg: Add 1ml → 2mg/ml. Ipamorelin 5mg: Add 2.5ml → 2mg/ml. Reconstitute separately. Both stable at 2–8°C for 28 days after reconstitution. Commonly used together in same injection.

Epithalon (10mg) Longevity

Add 1ml bac water → 10mg/ml. Epithalon dissolves quickly and forms a clear solution. Stable at 2–8°C for 28 days. Keep away from light after reconstitution.

GHK-Cu (50mg) Skin / Anti-Aging

Add 5ml bac water → 10mg/ml. GHK-Cu solution will appear blue or blue-green — this is normal due to copper binding. Stable at 2–8°C for 28 days.

NAD+ (500mg) Longevity / Energy

Add 5ml bac water → 100mg/ml. NAD+ dissolves quickly. Solution should be clear to light yellow. Keep refrigerated after reconstitution. Use within 14 days for best potency.

Semax / Selank (5mg) Cognitive

Add 1ml bac water → 5mg/ml. Both dissolve immediately. Colorless clear solution. Can also be diluted further with saline for intranasal use. Stable at 2–8°C for 28 days.

Common Questions

Can I use regular water instead of bacteriostatic water?

No. Regular water is not sterile and will cause bacterial growth. Sterile water (without benzyl alcohol) can be used short-term but peptides will degrade within 24–48 hours. Bacteriostatic water (0.9% benzyl alcohol) is required for storage.

My solution is slightly cloudy — is it ruined?

Slight cloudiness can occur with some peptides (especially TB-500) and usually clears with gentle swirling. Significant cloudiness or visible particles after full dissolution may indicate degradation or contamination — do not use.

How long is reconstituted peptide stable?

Generally 28 days at 2–8°C using bacteriostatic water. NAD+ degrades faster — use within 14 days. Unreconstituted lyophilized powder stored at −20°C is stable for 24+ months.

Can I mix two peptides in the same syringe?

Yes, in most cases — draw each into the syringe separately. Commonly combined: CJC-1295 + Ipamorelin, BPC-157 + TB-500. Reconstitute each separately first, then draw the desired amount from each vial into one syringe.

⚠ All products are sold for research purposes only. Not for human consumption. Not intended to diagnose, treat, cure, or prevent any disease or condition. This guide is for research reference only. Must be 18+ to purchase.
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